Massive sequencing laboratory

The Genomics area facilities are divided into two sections: the Molecular Biology laboratory, located in ITER’s main building; and the supercomputing infrastructure, located in the D-ALiX building.

The laboratory is divided into three compartmentalised rooms: the Molecular Biology laboratory itself, the sequencing room and the office area. Thus, the workflow in the Genomics area begins with sample preparation in the Molecular Biology laboratory, continues with mass sequencing in the sequencing room, and data processing on the teideHPC supercomputer, and ends in the office area, where the data are processed by the Genomics scientific team.

The equipment available in the sequencing laboratory is as follows:

  • 1 COVARIS M220 Focused-ultrasonicator (COVARIS Inc.)
  • 1 CFX96 Touch Real-Time PCR detection system (BioRad)
  • 2 Mastercycler pro S (Eppendorf)
  • 1 Qubit 3.0 Fluorometer (Thermo Fisher Scientific)
  • 1 Agilent 4200 TapeStation system (Agilent Technologies)
  • 1 NanoDrop One spectrophotometer (Thermo Scientific)
  • 1 cBot 2 System (Illumina, Inc.)
  • 1 MiSeq Sequencing system (Illumina, Inc.)
  • 1 NextSeq550 sequencing system (Illumina, Inc,)
  • 1 HiSeq 4000 sequencing system (Illumina, Inc.)
  • 1 Novaseq 6000 sequencing system (Illumina, Inc.)
  • 1 MinION sequencing system (Oxford Nanopore Technologies, Ltd.)
  • 1 MinION M1kC sequencing system (Oxford Nanopore Technologies, Ltd.)
  • 1 GridIONx5 sequencing system (Oxford Nanopore Technologies, Ltd.)
  • 1 Voltrax system (Oxford Nanopore Technologies, Ltd.)
  • 1 Freedom EVO100 automation system (TECAN)
GENÓMICA DATOS