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Sequencing services

  • Coverage options: minimum 30x.
  • Library preparation: Illumina DNA Prep or Illumina DNA PCR-Free Prep, among others.
  • Human WGS quality controls.
    • The sequenced samples are subject to stringent quality control protocols, including integrity analysis and analysis of nucleic acid concentration and removal of impurities.
    • In silico sex determination from sequencing data.
  • We perform de novo assembly for reference-free genomes.
  • Available on the NovaSeq 6000 sequencer (up to 28 complete human genomes per flow cell, 90 Gb/genome, >30x, 2×150 bp). Possibility to design experiments per flow cell and per line.
  • Low coverage whole genome sequencing (lcWGS) will soon be available on both second and third generation sequencers.

In the case of non-human WGS, please note that we can customise the preparation and sequencing of the organism of interest. Please contact us without obligation. Other species: viruses, bacteria, vascular plants, other mammals, etc.

  • Library preparation: Illumina DNA Prep with Exon 2.5 Enrichment.
  • We also have experience in using the Agilent SureSelect HS2 XT (v8) exome capture kit and adapting protocols to other insert size options.
  • Available on the NovaSeq 6000 sequencer for projects (up to 200 exomes at 100x with 2 x 100 bp reads).
    Possibility to design experiments per flow cell and per lane.
  • Available on the NextSeq 550 sequencer for pilot projects and projects with a small number of samples or urgent projects (6 exomes at 100x with 2 x 75 bp reads).
  • Our team has processed several thousand exomes from different national and foreign research centres and health institutions.
  • mRNA
    • We used oligo-dT beads to enrich the polyadenylated RNA molecules in the sample.
    • Sequencing is available on the NextSeq 550 and NovaSeq 6000, with read lengths of 75 bp and 100 bp in
      paired-end mode, respectively.
    • Library preparation protocols preserve chain information.
    • Library preparation: Illumina TruSeq Stranded mRNA.
  • Total RNA
    • Total RNA allows the study of protein-coding RNA as well as long non-coding RNAs.
    • Available read lengths are 75 bp and 100 bp in paired-end mode.
    • Depletion of ribosomes in the library preparation minimises the presence of rRNA.
    • Library preparation: Illumina Stranded Total RNA with Ribo-zero.
  • sRNA
    • Small RNA provides the ability to study RNA from 17 to 37 bp.
    • Study of a wide variety of classes of regulatory RNAs.
    • Library preparation: Illumina TruSeq Small RNA.
  • Of interest for the creation of random DNA fragments from the sample, e.g. from a clinical or environmental sample, which are subsequently sequenced and assembled to reconstruct the complete sequence.
  • Library preparation: Illumina Nextera XT and various options for long readings.
  • Available on the MiSeq, NextSeq 550 and NovaSeq 6000 platforms. Also available on nanopore-based sequencers (MinION Mk1B/Mk1C and GridION x5).
  • Possibility to design experiments per flow cell and per line.
  • Of interest to identify DNA-associated protein binding sites in the genome. This technique combines chromatin immunoprecipitation (ChIP) with bulk sequencing of DNA to provide a detailed picture of the interaction between proteins and DNA.
  • Library preparation: Illumina TruSeq ChIP Library Preparation Kit.
  • Of interest to identify certain epigenetic modifications (e.g methylation) of DNA that play a role in the regulation of gene expression and other cellular processes.
  • Library preparation: Twist Targeted Methylation.
  • Available on NovaSeq 6000. Possibility to design experiments per flow cell and per line.
  • Sequencing of whole genomes of bacteria and viruses.
  • Sequencing of 16S rRNA.
  • Preparation of libraries for viruses (e.g. SARS-CoV-2, influenza and respiratory syncytial virus): Illumina CovidSeq Test.
  • Available on MiSeq, NextSeq 550 and NovaSeq 6000. Possibility to design experiments per flow cell and per line.
  • Sequencing of whole genomes of bacteria and viruses.
  • Sequencing of 16S rRNA.
  • Adaptive sequencing (e.g. plasmid sequencing).
  • Metabarcoding (e.g. IOC sequencing).
  • Library preparation: consult ONT alternatives.
  • Available in low performance platforms (MinION Mk1B/Mk1C) and larger scale projects (GridION x5).

Description of the platforms

Sequencing services

Bioinformatics services

Access to services

High-throughput proteomics services

Conditions of services

Cost of services

Institutional Collaboration

CONTACT INFORMATION

Institute of Technology and Renewable Energies – ITER S.A.
Polígono Industrial de Granadilla, s/n
38600 – Granadilla de Abona
Santa Cruz de Tenerife – Spain

(+34) 922-747-700
difusion@iter.es

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